How to find sgRNA target sequences using CHOPCHOP

Hi sir,
I am fine.

Today, I introduce CHOPCHOP which is web-based search tool of CRISPR–Cas single guide RNA (sgRNA) targets.

Citation info: Labun, Kornel, et al. “CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing.” Nucleic acids research (2019)..

Thanks to many updates and author’s efforts, CHOPCHOP accommodates various sgRNA designs such as knock-out, knock-down, and knock-in…
In this post, I put a simple example. Please watch the following video.

I strongly recommend choosing the specific target where MM(MisMatch)0 is 0. And also, you can choose the target with MM1=0, MM2=0, and MM3=0 as long as you can.
CHOPCHOP can offer primer set for further analysis by deep sequencing or a T7E1 assay, and it can predict indel frequency based on Shen et al. 2018. It would be helpful for your experiment.

Thank you so much…