How to find sgRNA target sequences using CRISPOR

Writer: Kazuki Nakamae

Hi sir,
What are you planning in the winter vacation?

Today, I introduce CRISPOR that accommodates on/off-target scoring algorithms. And also CRISPOR can provide protocols, primer sequences, information of off-target sites, and analysis commands of CRISPResso, which meet the sgRNA you chose.

Citation info: Haeussler, Maximilian, et al. “Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR.” Genome biology 17.1 (2016): 148..

I uploaded the video. You can check it.

I usually enter the sequence name because an error occurs unless I enter it.
In this video, I used the VEGFA sequence of hg19. If you want to know how to get the information, you can check the previous article.

Thank you so much…