How to design knock-in donor using GTagHD

Hi sir,
How are you?

Today, I introduce GTagHD that helps knock-in design using short homology arms. This software can design knock-in donors even if you do not have sequence information of the interested genomic site.

Citation info: Wierson, Wesley A., et al. “GeneWeld: a method for efficient targeted integration directed by short homology.” bioRxiv (2018): 431627..
Note: I am NOT the author, just the user.

I uploaded the video. You can check it.

When you use it, the UCSC genome browser is helpful, because it can provide potential sgRNAs and RefSeq Gene ID of targeting gene. In this example, I design the knock-in donor sequence for VEGFA.

Thank you so much…