How to predict microhomology using Microhomology-Predictor

Hi sir,
Happy New Year!

Today, I introduce Microhomology-Predictor that predict microhomology site used for MMEJ, which estimate MMEJ-based deletion pattrns and frequency of frame-shift.

Citation info: Bae, Sangsu, et al. “Microhomology-based choice of Cas9 nuclease target sites.” Nature methods 11.7 (2014): 705..
Note: I am NOT the author, just the user.

I uploaded the video. You can check it.

Microhomology-Predictor resovgnizes the center of the entered sequence as cut site by RGEN (e.g. CRISPR-Cas9). And, the PAM-proximal region was right half-site in this example.

The result contains Microhomology Score and Out-of-frame Score. If these score are high, the frequencies of MMEJ and frame-shift are expected to become high.
If you want to get frame-shift mutation, a target where the Out-of-frame Score is >66 is better for that.

When you click the target, the predicted pattrns, including microhomology sequence and deletion length,will be shown.

Thank you so much…