How to analyze CRISPR-Cas9 NGS sample using Cas-Analyzer

Hi sir,
I have a headache. I took Pocari Sweat and the medicine. I wish to be better…

Anyway, today, I introduce Cas-analyzer which is a web-based NGS analysis tool. This is specialized for analyzing knock-out/knock-in data generated with CRISPR.

Citation info: Park J. et al. Cas-Analyzer: an online tool for assessing genome editing results using NGS data. Bioinformatics 33, 286-288 (2017).

I use the published filtered NGS amplicon data (qfltr_DRR147084_1.fastq / qfltr_DRR147084_2.fastq) which was generated in the step 6 of the post

The data is a knock-in sample of ATP5B locus in a human cell according to the paper. You can see the sequence infromation in the “Methods” block.

We show the analysis example. Please watch the following video.

Donor sequence is optional parameter. If you analize knock-out data, the information is not required.

As I showed the result in this video, the indel frequency is 61.0%. CrispRVariants showed that mutation efficiency of the same sample was 61.37% in the past post.
I feel that the algorithm of Cas-Analyzer is quite simple, but it is effective.

Thank you so much…